Company Overview:
HydroGene Therapeutics is a biotech startup specializing in cutting-edge gene therapy solutions for monogenic liver disorders. Our dedicated team of scientists is committed to advancing medical knowledge and transforming patient outcomes through innovative molecular interventions.

The Role:
We are seeking a highly skilled and motivated bench scientist with extensive hands-on experience in molecular biology and gene editing techniques. This individual will join the research team and play a key role in advancing our gene therapy research initiatives, contributing to the development of novel treatments for monogenic liver diseases. This is a full-time role that will be 100% on-site, with a majority of time spent on the research bench, and is based in Cambridge, MA.

Responsibilities:

Molecular Biology:
- Design molecular biology reagents for genome editing (knock-in, knock-out) and downstream assays (NGS, qPCR and, ddPCR primers/probes)
- Perform gel electrophoresis, Western blot, and DNA/RNA extraction from cells and tissues
- Conduct and troubleshoot polymerase chain reaction assays (PCR, qPCR, RT-PCR and ddPCR).
CRISPR genome editing
- Conduct work flows that include CRISPR editing cells in tissue culture (electroporation, lipid transfection) and assessing the downstream performance of the editing by a variety of methods (NGS, ddPCR, reporter assays)
Cell Biology:
- Cell culture: Cell line maintenance, transfection and electroporation, ideally with experience culturing primary hepatocytes
- In vitro cell based assays, experience with flow cytometry preferred
Project Management:
- Execute benchwork independently, demonstrating a high level of technical proficiency
- Organize all research data, maintain electronic lab notebook
- Manage research projects from conception to completion
Team Collaboration:
- Train and supervise junior laboratory technicians, fostering a collaborative and efficient work environment

Qualifications:

Ph.D. in a biomedical field with a focus on molecular biology/genetics/genome editing with up to 3 years of research experience in the pharmaceutical/biotech industry or post-doctoral experience in relevant field.
Proven expertise in molecular biology techniques, including DNA cloning, DNA/RNA extraction, qPCR, western blotting
Strong background in genome editing/engineering and associated techniques/assays
Ability to work independently, manage projects, and troubleshoot technical challenges
Excellent communication and interpersonal skills
Previous experience with mouse models is preferred

HydroGene Therapeutics is an equal opportunity employer. We celebrate diversity and are committed to creating an inclusive environment for all employees.

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